Gene disruption by transformation in Neurospora crassa.

Biotransformation of Hydrocortisone by Neurospora crassa

The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-h...

Activator-independent gene expression in Neurospora crassa.

A transgenic position effect that causes activator-independent gene expression has been described previously for three Neurospora crassa phosphate-repressible genes. We report analogous findings for two additional positively regulated genes, qa-2+ and ars-1+, indicating that such position effects are not limited to genes involved in phosphorus metabolism. In addition, we have characterized a nu...

Transformation of Aspergillus nidulans by the orotidine-5'-phosphate decarboxylase gene of Neurospora crassa.

Relief of an auxotrophic requirement for uridine in Aspergillus nidulans strain G191 has been achieved by transformation with a segment of Neurospora crassa DNA containing the corresponding gene coding for orotidine-5'-phosphate decarboxylase. The mitotic stability of such transformants suggests that the DNA has integrated into the genome. Southern hybridisation analysis of DNA isolated from tr...

biotransformation of hydrocortisone by neurospora crassa

the ability of neurospora crassa fgsc 4335 in the biotransformation of hydrocortisone was investigated. the microorganism produced two major metabolites after incubation with the substrate for seven days. each microbial product was purified chromatographically and identified on the basis of spectral data. the products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (ii) and 11?-h...

Quelling in Neurospora crassa.